Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100026 |
| Name | oriT_pK214  |
| Organism | Lactococcus lactis subsp. lactis K214 |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | NC_009751 (_) |
| oriT length | 10 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 7..8 |
| Conserved sequence flanking the nic site |
TAGTGTG|TTA |
| Note | from GenBank |
oriT sequence
Download Length: 10 nt
>oriT_pK214
TAGTGTGTTA
TAGTGTGTTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Hickey RM et al. (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]
Relaxase
| ID | 25 | GenBank | CAA63521 |
| Name | Mob_pK214 |
UniProt ID | O32791 |
| Length | 403 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 403 a.a. Molecular weight: 46815.76 Da Isoelectric Point: 9.2672
>CAA63521.1 mobilization protein (plasmid) [Lactococcus lactis subsp. lactis K214]
MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFN
DEHKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKS
EVLALSNDKAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYE
ELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLKNEIKGIYKGF
NQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRDELSR
MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFN
DEHKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKS
EVLALSNDKAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYE
ELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLKNEIKGIYKGF
NQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRDELSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | O32791 |
Reference
[1] Hickey RM et al. (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]
[2] Perreten V et al. (1997) Antibiotic resistance spread in food. Nature. 389(6653):801-2. [PMID:9349809]
Host bacterium
| ID | 25 | GenBank | NC_009751 |
| Plasmid name | pK214 | Incompatibility group | _ |
| Plasmid size | 29871 bp | Coordinate of oriT [Strand] | 7087..7098 [+] |
| Host baterium | Lactococcus lactis subsp. lactis K214 |
Cargo genes
| Drug resistance gene | resistance to tetracyline, chloramphenicol, and streptomycin |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |