Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100025
Name   oriT_pS7a experimental
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis pS7
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   AJ550509 (415..870 [+], 456 nt)
oriT length   456 nt
IRs (inverted repeats)      IR1: 132..144, 150..161  (TGCCCATTTTTAT..ATAAAATGGGCA)
  IR2: 205..217, 221..233  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 240..253, 266..279  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      288..289
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   Lactococcal plasmid

  oriT sequence  


Download         Length: 456 nt

>oriT_pS7a
GGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGAAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Strahinic I et al. (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]


Host bacterium

ID   24 GenBank   AJ550509
Plasmid name   pS7a Incompatibility group   _
Plasmid size   7302 bp Coordinate of oriT [Strand]   415..870 [+]
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis pS7

Cargo genes

Drug resistance gene   _
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -