Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100024
Name   oriT_pS7b experimental
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis pS7b
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   AJ550510 (415..870 [+], 456 nt)
oriT length   456 nt
IRs (inverted repeats)      IR1: 133..145, 151..162  (TGCCCATTTTTAT..ATAAAATGGGCA)
  IR2: 206..218, 222..234  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
 IR3: 241..254, 267..280  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      289..290
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   Lactococcal plasmid

  oriT sequence  


Download         Length: 456 nt

>oriT_pS7b
TGGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTTATTTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGAGTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Strahinic I et al. (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]


Host bacterium

ID   23 GenBank   AJ550510
Plasmid name   pS7b Incompatibility group   _
Plasmid size   7264 bp Coordinate of oriT [Strand]   415..870 [+]
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis pS7b

Cargo genes

Drug resistance gene   _
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -