Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100023
• Name: oriT_pS6
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis pS6
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): AJ550511 ( , 482 nt)
• oriT length: 482 nt
• IRs (inverted repeats): IR1: 21..33, 37..49 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR2: 55..69, 82..96 (CACATTGTAATACAA..TTGTATTACAATGTG)
• Location of nic site: 104..105
• Conserved sequence flanking the nic site: CTTG|CA
• Note: _

oriT sequence

• Length: 482 nt

>oriT_pS6
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAAATCAAACAAAGACTCACTCATTTAAGAGATGCTACTATCATGAAATTTTGTTGTTGCGATAAGCAACTTCTAATACACGATTTTTAGCCATTACATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGCCGACCAGCGTTTGAAACTCTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTCGA

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Strahinic I et al. (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]

Host bacterium

• ID: 22
• GenBank: AJ550511
• Plasmid name: pS6
• Incompatibility group: _
• Plasmid size: 482 bp
• Coordinate of oriT [Strand]: _
• Host baterium: Lactococcus lactis subsp. lactis bv. diacetylactis pS6

Cargo genes

• Drug resistance gene: _
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -