Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100022
Name   oriT_pS80 in_silico
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis pS80
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   AJ550512 ( , 604 nt)
oriT length   604 nt
IRs (inverted repeats)      IR1: 141..153, 157..169  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR2: 176..189, 202..215  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      224..225
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   _

  oriT sequence  


Download         Length: 604 nt

>oriT_pS80
TCGAGCCCTTCCCGAGCCATGGTCAAGCGCCAGGGCGGAACATTCTTGCGAGCACAAGGACGTGGGATATACCTACGCTACCTCCCTATCAGCGATCGGGGCCGTGGCTGGAAAGCAATCCGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTAGTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAGTACATTCAATTTAGATTGTCTGAAGAACAATACAATAAGCTGAAAATTTCAGGAGAAACATATGGACTCTCTCCGAATCTTTATGCGAAAAAATTAGCACAAAAAATCTCATTTAAAAAACCTTATCTCGGCTGAATTACTGGGTTCACAGCGGATGAAGGATTTCCTAAAGTCAGCTAAGGAACGGTATGACATTGTGATTGTCGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Strahinic I et al. (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]


Host bacterium

ID   21 GenBank   AJ550512
Plasmid name   pS80 Incompatibility group   _
Plasmid size   604 bp Coordinate of oriT [Strand]   _
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis pS80

Cargo genes

Drug resistance gene   _
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -