Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100020 |
| Name | oriT_pLME300  |
| Organism | Limosilactobacillus fermentum |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_004566 (16740..17048 [+], 309 nt) |
| oriT length | 309 nt |
| IRs (inverted repeats) | IR1: 1..10, 11..20 (ATATTGGTAT..AGACCAATAT) IR2: 108..116, 119..127 (ATAAAATTT..AAATTTTAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | _ |
oriT sequence
Download Length: 309 nt
>oriT_pLME300
ATATTGGTATAGACCAATATTGGTCTAATGTTAAACATTGTTTTTGAACCGACGAATTACACGATTTAATCGAACGTTTTGACCCATTGGTCTAGTTCGAAAATCGTATAAAATTTCAAAATTTTATCAATGAGGAGGTCCTTGTTTATGCCCGAAGTTAAACGAAACTTGATTCAGGTGCGACTAACCGACGCACAGCTAAGTGAGTTTGATGATGTTAAGAAGTTGTTGCATGTTAGCACTAGTGCTGATGCTCTTCGAAAACTAATTAATGATAAGAAGCTAACCAGTGGTGTGACCCAAGAAGCT
ATATTGGTATAGACCAATATTGGTCTAATGTTAAACATTGTTTTTGAACCGACGAATTACACGATTTAATCGAACGTTTTGACCCATTGGTCTAGTTCGAAAATCGTATAAAATTTCAAAATTTTATCAATGAGGAGGTCCTTGTTTATGCCCGAAGTTAAACGAAACTTGATTCAGGTGCGACTAACCGACGCACAGCTAAGTGAGTTTGATGATGTTAAGAAGTTGTTGCATGTTAGCACTAGTGCTGATGCTCTTCGAAAACTAATTAATGATAAGAAGCTAACCAGTGGTGTGACCCAAGAAGCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Gfeller KY et al. (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]
Relaxase
| ID | 19 | GenBank | NP_783849 |
| Name | MobA_pLME300 |
UniProt ID | Q84EV8 |
| Length | 426 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 426 a.a. Molecular weight: 48783.87 Da Isoelectric Point: 10.7856
>NP_783849.1 putative mobilization protein MobA (plasmid) [Lactobacillus fermentum]
MATLMSNACRSSYGRLTYVFNQSAHNTTKTSHRVLASSGINIKMLHNPDGTLSLVQSGAYLEKQFHNSLK
RAFNPKRKIQCQSIIISFSNNEFDTTNLSEQASQALQLVQGYANEFFDDAQSVSVVQADGNGGRLHVHLL
INAVNPSGRTIATNRFSVYKMRRNLNDYLNHNFERVTGRKWENPFEKTENRKDLDNLSSRSIWQEQLKKT
IALVKTEVDNVKDFLNKLSQLGITVTERQQGKSWTYHSTIKTAKGTKKVSARDFYQRIDKNTGQVLTTRG
LGQAYTKQSLEAYYQRMLSKQKEILPLETPPHHTRKEVDNNVRNKEEREQLAKIKTRAAEARAQITRQRE
INQLNLRQLAAAKAEEQRQQQQAERKAGQAHRQQSHGKHAQSIIDQERLRKRAEQRLRAKQQNAKSSRSK
DAGPDL
MATLMSNACRSSYGRLTYVFNQSAHNTTKTSHRVLASSGINIKMLHNPDGTLSLVQSGAYLEKQFHNSLK
RAFNPKRKIQCQSIIISFSNNEFDTTNLSEQASQALQLVQGYANEFFDDAQSVSVVQADGNGGRLHVHLL
INAVNPSGRTIATNRFSVYKMRRNLNDYLNHNFERVTGRKWENPFEKTENRKDLDNLSSRSIWQEQLKKT
IALVKTEVDNVKDFLNKLSQLGITVTERQQGKSWTYHSTIKTAKGTKKVSARDFYQRIDKNTGQVLTTRG
LGQAYTKQSLEAYYQRMLSKQKEILPLETPPHHTRKEVDNNVRNKEEREQLAKIKTRAAEARAQITRQRE
INQLNLRQLAAAKAEEQRQQQQAERKAGQAHRQQSHGKHAQSIIDQERLRKRAEQRLRAKQQNAKSSRSK
DAGPDL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q84EV8 |
Reference
[1] Gfeller KY et al. (2003) Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid. 50(3):190-201. [PMID:14597008]
Host bacterium
| ID | 19 | GenBank | NC_004566 |
| Plasmid name | pLME300 | Incompatibility group | _ |
| Plasmid size | 19389 bp | Coordinate of oriT [Strand] | 16740..17048 [+] |
| Host baterium | Limosilactobacillus fermentum |
Cargo genes
| Drug resistance gene | resistance to streptogramin A and erythromycin |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |