Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100018 |
| Name | oriT_pSU316  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | X55894 ( , 279 nt) |
| oriT length | 279 nt |
| IRs (inverted repeats) | 220..227, 230..237 (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 246..247 |
| Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
| Note | _ |
oriT sequence
Download Length: 279 nt
>oriT_pSU316
CGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
CGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Salazar L et al. (1992) Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233. Mol Gen Genet . 234(3):442-8. [PMID:1406590]
Auxiliary protein
| ID | 27 | GenBank | CAA39377 |
| Name | TraM_pSU316 |
UniProt ID | Q47544 |
| Length | 127 a.a. | PDB ID | _ |
| Note | _ | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14577.58 Da Isoelectric Point: 5.0516
>CAA39377.1 TraM (plasmid) [Escherichia coli]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKEFAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKEFAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q47544 |
Reference
[1] Salazar L et al. (1992) Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233. Mol Gen Genet . 234(3):442-8. [PMID:1406590]
Host bacterium
| ID | 17 | GenBank | X55894 |
| Plasmid name | pSU316 | Incompatibility group | IncFIII/IV |
| Plasmid size | 1078 bp | Coordinate of oriT [Strand] | _ |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |