oriTDB
I. Information of Relaxase
ID 6
Name TraI_pED208 experimental
GenBank accession number AAM90727
Family MOBF
Length 1767 aa
UniProt ID Q8KNJ9
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.20E-71, Aligned region: 7..280
TraI [PF07057.10], Evalue: 4.60E-46, Aligned region: 1441..1562
AAA_30 [PF13604.5], Evalue: 2.50E-34, Aligned region: 970..1162
AAA_19 [PF13245.5], Evalue: 7.80E-10, Aligned region: 975..1111
Note OriT nicking and unwinding; similar to TraI of plasmid F
Protein sequence [Download] MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG
SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALIGEDRRFIEAHNRAVAVVMQEVEQLVSA
RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT
GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE
AAGPDASAKSRDVAALDTRQAKAWADPDLLKAEWRRRLTDEKFDIDNYISQAQARVEVSG
PVVAGQGGISASGQPGIASSGEAADELVQKAVGDTISALSDKKVQFTWSEMLAGTVSRLP
SAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF
PERAQERNIPAGDAVSVLSQDKSPVAILSCRGGAQALRERTEDVAMMARSQGREVMVIAA
DGRSGQFLSESPQLAGQVMLRSQINADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG
AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLVSEADKRSRYSQLAQDYVR
LSAEGRDVVAQVTGTREQQQLTEIIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN
YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT
LEVSEGDRVRALGRELKGAIKAKEQFTVAGLENGAVRLRSGDRELRLPTERAVKLTHDYV
EGTGAGTSASRTVLAAVGPRGLNKQTLNALAQSGSDIRIYTPLTPEQAARKVESVSAVRL
ASDQVRQSTGEANLDTAIQASRERLMSDAEQAVNLAIPSAQQGQVYLSELTLLAEAVKSG
QPLADVRAEIARQVDSGALIKLDSVAGAGNRVLVPRVAYEMEKTIIRHIAEGKEAVQPLM
ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP
QVIGLGPTHRAVHEMREAGVDARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR
DMAELYQLVAAGGGRMVSSGDTAQLQAISTGLPFRLVQQRSAIDTVVMQEIVRQTPALRP
AIESIIAGQVEISLRQVDDVTPQQVPRQPGAWVPDNSVMEIRAPKKEQDQVQNIPVAGEQ
TLTPEQLALVRTDIIEAIRDDWMGRTPEAQQQTLVVAELNADRHAINEAIHTARHEKGDT
GAEERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADG
ESVLISPQQNTAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDEGAIR
FRQGDQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTY
VTLSRAKEHVQVYTDDLAGWSADARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDK
TALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPAWTRHGKAAGALLTEIRIEDDGMR
VVLSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLL
NAARLTGGRITEPDEVERTVRSVAEAESAAKAEDPITLPPDEQQKLAEAQEKAAQELAEQ
ARQDLLPALPGTEGDKPDPLLSASDERRLRDGTERETRELDEAVREAVAEGREPRQQVRD
QMQRTEREWVVNVPEKEIELEKTLGGD
Reference
[1] Lu J et al (2002) Analysis and characterization of the IncFV plasmid pED208 transfer region. Plasmid. 48(1):24-37. [PMID:12206753]
[2] Di Laurenzio L et al (1991) Characterization of the oriT region of the IncFV plasmid pED208. Mol Microbiol. 5(7):1779-90. [PMID:1943709]

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 11 TraM_pED208 experimental CAA42174 127 aa TraM mediates signalling between transferosome and relaxosome
2 12 TraY_pED208 experimental CAA42176 20 aa _