oriTDB
I. Information of Relaxase
ID 35
Name TraI_pEST4011 insolico
GenBank accession number NP_990929
Family MOBP
Length 730 aa
UniProt ID Q6QHL4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.70E-54, Aligned region: 12..252
Note relaxase
Protein sequence [Download] MIAKHVSMKTVQKSDFAGLVKYITDEQAKNERVGYVAVTNCHTDRPEVAITEVLNTQAQN
TRSGADKTYHLIVSFRPGEQPDDATLKAIEARICEGLGFGEHQRVSAVHHDTDNLHIHIA
INKIHPTRYTILEPFNAYHTLGQLCEKLEREYSLERDNHQGNKLVSEGRAQDMERHASVE
SLQGWVKRECAADMQAAQSWDALHQVMRDNGLELRERGNGFVIQAADGLDVKASSIGREF
SKAKLEERLGTFEPSAEHLERAAQKPRKQYDKKPVRSRVNTVELHAKYKADQLTISGSRT
AEWEKARAKKDRLIEAAKRSGRLKRSTIKLISGAGVGKKLMYSATSKTMKSEIEKINKQY
LKERQEIHEKYQRRAWADWLRAKAAEGDQEALGALRARDAAQGLKGNTVAGTGPLRPQAA
AAEQDSVTKKGTIIYRVGPTAVRDDGDRLKVSRGADQDGMQAALRMAMDRYGDRISVGGT
PAFKEQIAQAAAASKLPITFDDDALERRRQELLTQATTKENDHDRTDRGRAAGGGISGSG
PAIAAIPGAARAGQPRAAAAGRTTKPNVGRVGRKPPPQSQNRLRRLSQLGVVQLASGSEV
LLPRHVPGDVEQQGAKPVDGLRRDIFGPGRLSPQVAAAADKYIAEREGKRLKGFDIPKHS
RYTDGQDGAATFAGIRQVEGQSLALLTRGEEVMVLPVDEPTARRLKRLAVGDAITVTPQG
SIKTTKGRSR
Reference
[1] Vedler E et al (2004) The completely sequenced plasmid pEST4011 contains a novel IncP1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation. J Bacteriol. 186(21):7161-74. [PMID:15489427]

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 53 TraH_pEST4011 insolico NP_990930 108 aa relaxosome stabilization
2 54 TraJ_pEST4011 insolico NP_990932 129 aa oriT recognizing protein; required for relaxase activity
3 55 TraK_pEST4011 insolico NP_990933 132 aa oriT-binding protein; constitutes a part of relaxosome, necessary for nicking at oriT