oriTDB

oriT
Relaxosome
T4CP
Element&Host

The information of the oriT region

oriTDB accession number	200054
Name	oriT_Tn916(RST11)
Sequence Completeness	intact
oriT length	215 nt
oriT sequence	AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)	[61-76] [80-95] nt (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) [123-134] [139-150] nt (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site	[135-136] nt
Conserved sequence flanking the nic site	TGG\|T
Note	blastn alignment with the oriT_Tn916
	L:61-76;R:80-95;L:123-134;R:139-150;D:135-136

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	268
Name	ORF20_Tn916(RST11)
GenBank accession number	CBE66529
Family	MOB_T
Length	329 aa
UniProt ID	C8ZL35
PDB ID	_
Pfam	Rep_trans [PF02486.18], Evalue: 1.80E-69, Aligned region: 96..301
Note	putative transcriptional regulator
Protein sequence [Download]	MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI LDHAKLTDRHKQILKQQSVKEQDVITTKK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	357	_

Information of T4CP

ID	268
Name	ORF21_Tn916(RST11)
SecReT4 accesion number	_
GenBank accession number	CBE66528
Family	TrwB/TraD
Length	461
UniProt ID	C8ZL34
PDB ID	_
Pfam	FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313 DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note	_
Protein sequence [Download]

Reference
N/A

Information of mobile element

ID	59
Element type	Tn916-like
ICE description	Tn916(RST11)
ICEberg accesion number	307
GenBank accession number	FN550102.1
Family	Tn916
Genome size	18032 bp
Coordinate of oriT [+/-]	2441..2655 [+]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus rostri RST11 [522262]

Reference

[1] Stegmann R et al (2010) Antibiotic resistance profile of Staphylococcus rostri, a new species isolated from healthy pigs. Vet Microbiol. 145(1-2):165-71. [PMID:20399039]