oriTDB
The information of the oriT region
oriTDB accession number 200050
Name oriT_ICEFal35896-1  insolico
Sequence Completeness intact
oriT length 214 nt
oriT sequence AAGCGAAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCGATAGCCACGCCAGC ACCTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAATAG CAGAAAATTCTTTGGTTACAAGGAGTTTAGAAAATTTCGTGGTATGTCAAATGAGCTTCA ATAGTTGACATACCTTTTTTAATTGGAGGGATTT
IR (inverted repeat)[66-76] [80-89] nt  (ACCCCCCGTAT..ACAGGGGGGT)
[125-134] [139-148] nt  ( AAATTCTTTG..CAAGGAGTTT)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:66-76;R:80-89;L:125-134;R:139-148;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 264
Name Relaxase_ICEFal35896-1 insolico
GenBank accession number EFE28724
Family MOBT
Length 391 aa
UniProt ID _
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 5.80E-68, Aligned region: 158..363
HTH_3 [PF01381.21], Evalue: 7.60E-09, Aligned region: 5..47
Note DNA-binding helix-turn-helix protein
Protein sequence [Download] MIQHLKEKRLAYGLSQNRLAIATGITRQYLSDIETGKVKPSDELQQSLWETLERFNPDAP
LEMLFDYVRIRFPTMDVQHVVEEILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHEID
KGVLVELKGRGCRQFESYLLAQQRSWYEFFMDALVAGGVMKRLDLAINDKTGILNIPTLT
EKCLQEECISVFRSFKSYRSGELVRKDEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKN
DIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKARSD
WKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAITLDEINQTQVVK
DILDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 353 _ insolico
Information of T4CP
ID 264
Name T4CP_ICEFal35896-1 insolico
SecReT4 accesion number _
GenBank accession number EFE28725
Family TrwB/TraD
Length 464
UniProt ID _
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 1.40E-07, Aligned region: 210..317
DUF87 [PF01935.16], Evalue: 5.80E-05, Aligned region: 219..356
Note FtsK/SpoIIIE family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 55
Element type _
ICE description ICEFal35896-1
ICEberg accesion number 327
GenBank accession number CP002390.1
Family Tn916
Genome size 17986 bp
Coordinate of oriT  [+/-] 1262384..1262597 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Filifactor alocis ATCC 35896 [546269]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU