oriTDB
The information of the oriT region
oriTDB accession number 200049
Name oriT_Tn2010  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 263
Name ORF20_Tn2010 insolico
GenBank accession number BAG12482
Family MOBT
Length 348 aa
UniProt ID B1B630
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 2.10E-69, Aligned region: 96..301
Note similar to ORF20 in Tn6002
Protein sequence [Download] MLFDYVRIRFPTTDVQHVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG
VLVELKGRGCRQFESYLLAQQRSWYEFFMDALVAGGVMKRLDLAINDKTGILNIPVLTEK
CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI
PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK
LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI
LDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKRYNGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 352 _ insolico
Information of T4CP
ID 263
Name ORF21_Tn2010 insolico
SecReT4 accesion number _
GenBank accession number BAG12481
Family TrwB/TraD
Length 461
UniProt ID B1B629
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.60E-07, Aligned region: 206..312
DUF87 [PF01935.16], Evalue: 4.30E-05, Aligned region: 214..352
Note similar to ORF21 in Tn6002
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 54
Element type Tn916-like transposon
ICE description Tn2010
ICEberg accesion number 325
GenBank accession number AB426620.1
Family Tn916
Genome size 26390 bp
Coordinate of oriT  [+/-] 2441..2655 [+]
Drug resistance insolico erythromycin and tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae 05P294 [1313]
Reference
[1] Li Y et al (2011) Molecular characterization of erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010. J Appl Microbiol. 110(1):254-65. [PMID:20961364]

Microbial Bioinformatics Group in MML, SJTU