oriTDB
The information of the oriT region
oriTDB accession number 200048
Name oriT_ICESpnTCH8341-1  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 262
Name Relaxase_ICESpnTCH8341-1 insolico
GenBank accession number ADI70423
Family MOBT
Length 420 aa
UniProt ID D6ZP77
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.50E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 2.30E-10, Aligned region: 15..64
HTH_31 [PF13560.5], Evalue: 4.60E-07, Aligned region: 11..69
Note DNA-binding helix-turn-helix protein
Protein sequence [Download] MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLFE
ALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDALVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKRYNGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 351 _ insolico
Information of T4CP
ID 262
Name T4CP_ICESpnTCH8341-1 insolico
SecReT4 accesion number _
GenBank accession number ADI70421
Family TrwB/TraD
Length 461
UniProt ID D6ZP75
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.60E-07, Aligned region: 206..312
DUF87 [PF01935.16], Evalue: 4.30E-05, Aligned region: 214..352
Note FtsK/SpoIIIE family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 53
Element type Tn916-like
ICE description ICESpnTCH8341-1
ICEberg accesion number 324
GenBank accession number CP001993.1
Family Tn916
Genome size 26390 bp
Coordinate of oriT  [+/-] 2007439..2007653 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae TCH8431/19A [525381]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU