oriTDB
The information of the oriT region
oriTDB accession number 200047
Name oriT_ICECdiM120-1  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 261
Name Relaxase_ICECdiM120-1 insolico
GenBank accession number WP_000398284
Family Other
Length 401 aa
UniProt ID _
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 4.30E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.10E-06, Aligned region: 11..69
Note replication initiation protein
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 350 _ insolico
Information of T4CP
ID 261
Name T4CP_ICECdiM120-1 insolico
SecReT4 accesion number _
GenBank accession number WP_000813488
Family TrwB/TraD
Length 461
UniProt ID _
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note cell division protein FtsK
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 52
Element type Tn916-like
ICE description ICECdiM120-1
ICEberg accesion number 323
GenBank accession number NC_017174.1
Family Tn916
Genome size 17902 bp
Coordinate of oriT  [+/-] 2186896..2187110 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Clostridium difficile M120 [699035]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU