oriTDB
The information of the oriT region
oriTDB accession number 200046
Name oriT_Tn6003  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 260
Name ORF20FTn6003 insolico
GenBank accession number CAL92505
Family MOBT
Length 400 aa
UniProt ID A4F5E2
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 96..301
Omega_Repress [PF07764.10], Evalue: 4.90E-31, Aligned region: 333..392
Note _
Protein sequence [Download] MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG
VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK
CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI
PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK
LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI
LDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHI
IKIETAKNGGNVKEVMEIRLRSKLKSVLIVQYLKILYNRN
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 349 _ insolico
Information of T4CP
ID 260
Name ORF21_Tn6003 insolico
SecReT4 accesion number _
GenBank accession number CAP45529
Family TrwB/TraD
Length 461
UniProt ID A9J066
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note _
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 51
Element type Tn916-like
ICE description Tn6003
ICEberg accesion number 321
GenBank accession number AM410044.5
Family Tn916
Genome size 25101 bp
Coordinate of oriT  [+/-] 2441..2655 [+]
Drug resistance experimental resistance to erythromycin and tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae Ar4 [1313]
Reference
[1] Cochetti I et al (2007) New Tn916-related elements causing erm(B)-mediated erythromycin resistance in tetracycline-susceptible pneumococci. J Antimicrob Chemother. 60(1):127-31. [PMID:17483548]

Microbial Bioinformatics Group in MML, SJTU