oriTDB
The information of the oriT region
oriTDB accession number 200040
Name oriT_ICECdiM68-1  insolico
Sequence Completeness intact
oriT length 184 nt
oriT sequence GCTGGTGTGGCGTAAGCCACGCCAGCAGGTAAAACCCCTCGGTATCTAACAGGGGGGTAC AAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACA AAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGATTGGAGGG ATTT
IR (inverted repeat)[1-11] [16-26] nt  (GCTGGTGTGGC..GCCACGCCAGC)
[92-103] [108-119] nt  ( GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [104-105] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:1-11;R:16-26;L:92-103;R:108-119;D: 104-105
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 254
Name Relaxase_ICECdiM68-1 insolico
GenBank accession number WP_000398284
Family MOBT
Length 401 aa
UniProt ID _
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 4.30E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.10E-06, Aligned region: 11..69
Note _
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 343 _ insolico
Information of T4CP
ID 254
Name T4CP_ICECdiM68-1 insolico
SecReT4 accesion number _
GenBank accession number WP_044593066
Family TrwB/TraD
Length 464
UniProt ID _
PDB ID _
Pfam
Note cell division protein FtsK
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 45
Element type Tn916-like
ICE description ICECdiM68-1
ICEberg accesion number 314
GenBank accession number NC_017175.1
Family Tn916
Genome size 18691 bp
Coordinate of oriT  [+/-] 337912..338095 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Clostridium difficile M68 [699037]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU