oriTDB
The information of the oriT region
oriTDB accession number 200035
Name oriT_ICESpaNUF1049  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 249
Name Relaxase_ICESpaNUF1049 insolico
GenBank accession number BAG80620
Family MOBT
Length 329 aa
UniProt ID B6F268
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 1.80E-69, Aligned region: 96..301
Note similar to Tn916(ORF20); U09422
Protein sequence [Download] MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG
VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK
CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI
PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK
LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI
LDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 338 _ insolico
Information of T4CP
ID 249
Name T4CP_ICESpaNUF1049 insolico
SecReT4 accesion number _
GenBank accession number BAG80619
Family TrwB/TraD
Length 461
UniProt ID B6F267
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note similar to Tn916(ORF21);U09422
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 40
Element type Streptococcus parauberis DNA, Tn916-like element and flanking region
ICE description ICESpaNUF1049
ICEberg accesion number 308
GenBank accession number AB468159.1
Family Tn916
Genome size 18031 bp
Coordinate of oriT  [+/-] 5456..5670 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus parauberis NUF1049 [1348]
Reference
[1] Meng F et al (2009) Structural characterization of Tn916-like element in Streptococcus parauberis serotype II strains isolated from diseased Japanese flounder. Lett Appl Microbiol. 48(6):770-6. [PMID:19344360]

Microbial Bioinformatics Group in MML, SJTU