oriTDB
The information of the oriT region
oriTDB accession number 200032
Name oriT_ICESga43143-1  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site
TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 246
Name Relaxase_ICESga43143-1 insolico
GenBank accession number BAK28456
Family MOBT
Length 401 aa
UniProt ID F5WVZ9
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 4.30E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.10E-06, Aligned region: 11..69
Note Tn916 ORF20 phage replication initiation factor PEP
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 335 _ insolico
Information of T4CP
ID 246
Name T4CP_ICESga43143-1 insolico
SecReT4 accesion number _
GenBank accession number BAK28457
Family TrwB/TraD
Length 461
UniProt ID F5WW00
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note Tn916 ORF21 FtsK/SpoIIIE family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 37
Element type Tn916-like transposable element
ICE description ICESga43143-1
ICEberg accesion number 304
GenBank accession number AP012053.1
Family Tn916
Genome size 18032 bp
Coordinate of oriT  [+/-] 1658753..1658967 [-]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus gallolyticus subsp. gallolyticus ATCC 43143 [981539]
Reference
[1] Lin IH et al (2011) Sequencing and comparative genome analysis of two pathogenic Streptococcus gallolyticus subspecies: genome plasticity, adaptation and virulence. PLoS One. 6(5):e20519. [PMID:21633709]