oriTDB
The information of the oriT region
oriTDB accession number 200031
Name oriT_Tn6009  insolico
Sequence Completeness intact
oriT length 216 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 245
Name Relaxase_Tn6009 insolico
GenBank accession number ACF08822
Family MOBT
Length 329 aa
UniProt ID B5SU30
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 1.80E-69, Aligned region: 96..301
Note similar to Tn916 Orf20 in NC_006372
Protein sequence [Download] MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG
VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK
CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI
PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK
LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI
LDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 334 _ insolico
Information of T4CP
ID 245
Name T4CP_Tn6009 insolico
SecReT4 accesion number _
GenBank accession number ACF08821
Family TrwB/TraD
Length 461
UniProt ID B5SU29
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note similar to Tn916 Orf21 in NC_006372
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 36
Element type EU239355.1 (transposon Tn6009, partial sequence)
ICE description Tn6009
ICEberg accesion number 104
GenBank accession number EU239355.1
Family Tn916
Genome size _
Coordinate of oriT  [+/-] 5994..6209 [+]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae strain 41 [573]
Reference
[1] Soge OO et al (2008) A novel transposon, Tn6009, composed of a Tn916 element linked with a Staphylococcus aureus mer operon. J Antimicrob Chemother. 62(4):674-80. [PMID:18583328]

Microbial Bioinformatics Group in MML, SJTU