oriTDB
The information of the oriT region
oriTDB accession number 200029
Name oriT_ICESpn9409  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTAAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 243
Name Orf20_ICESpn9409 insolico
GenBank accession number CBW39427
Family MOBT
Length 420 aa
UniProt ID E8ZEI5
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.50E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 4.60E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.20E-06, Aligned region: 11..69
Note _
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKRYNGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 332 _ insolico
Information of T4CP
ID 243
Name Orf21_ICESpn9409 insolico
SecReT4 accesion number _
GenBank accession number CBW39426
Family TrwB/TraD
Length 461
UniProt ID B7UTY5
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note conjugative transposon FtsK/SpoIIIE-family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 34
Element type Tn916-type integrative and conjugative element
ICE description ICESpn9409
ICEberg accesion number 87
GenBank accession number FR671418.1
Family Tn916
Genome size 22765 bp
Coordinate of oriT  [+/-] 2106..2320 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae 9409 [1313]
Reference
[1] Croucher NJ et al (2011) Rapid pneumococcal evolution in response to clinical interventions. Science. 331(6016):430-4. [PMID:21273480]

Microbial Bioinformatics Group in MML, SJTU