oriTDB
The information of the oriT region
oriTDB accession number 200025
Name oriT_ICESpnH034800032  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 239
Name Orf20_ICESpnH034800032 insolico
GenBank accession number CBW39343
Family MOBT
Length 472 aa
UniProt ID B7UTY4
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 4.60E-69, Aligned region: 168..373
Omega_Repress [PF07764.10], Evalue: 2.20E-30, Aligned region: 405..464
HTH_3 [PF01381.21], Evalue: 5.30E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.40E-06, Aligned region: 11..69
Note _
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGD
KTVRVRADLHHIIKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 328 _ insolico
Information of T4CP
ID 239
Name Orf21_ICESpnH034800032 insolico
SecReT4 accesion number _
GenBank accession number CBW39342
Family TrwB/TraD
Length 461
UniProt ID B7UTY5
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note conjugative transposon FtsK/SpoIIIE-family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 30
Element type Tn916-type integrative and conjugative element
ICE description ICESpnH034800032
ICEberg accesion number 82
GenBank accession number FR671414.1
Family Tn916
Genome size 20370 bp
Coordinate of oriT  [+/-] 2106..2320 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae H034800032 [1313]
Reference
[1] Croucher NJ et al (2011) Rapid pneumococcal evolution in response to clinical interventions. Science. 331(6016):430-4. [PMID:21273480]

Microbial Bioinformatics Group in MML, SJTU