oriTDB
The information of the oriT region
oriTDB accession number 200023
Name oriT_CTn6002  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site
TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 237
Name ORF20_CTn6002 insolico
GenBank accession number AAY63930
Family MOBT
Length 400 aa
UniProt ID Q3KU49
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 96..301
Omega_Repress [PF07764.10], Evalue: 1.70E-30, Aligned region: 333..392
Note ORF20F; similar to ORF20 in Tn916, YP_133675, but extended by 72 amino acids
Protein sequence [Download] MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKG
VLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEK
CQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDI
PIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWK
LNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDI
LDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHI
IKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 326 _ insolico
Information of T4CP
ID 237
Name ORF21_CTn6002 insolico
SecReT4 accesion number _
GenBank accession number AAY63929
Family TrwB/TraD
Length 461
UniProt ID Q3KU50
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note ORF21; similar to ORF21 in Tn916, YP_133674, and DNA segregation ATPase, FtsK/SpoIIIE family protein, ZP_00285890
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 28
Element type Tn916-like transposon
ICE description CTn6002
ICEberg accesion number 62
GenBank accession number AY898750.1
Family Tn916
Genome size 20880 bp
Coordinate of oriT  [+/-] 2441..2655 [+]
Drug resistance insolico tetracycline resistance; erythromycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus cristatus [45634]
Reference
[1] Warburton PJ et al (2007) Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon. J Antimicrob Chemother. 60(5):973-80. [PMID:17855723]