oriTDB
The information of the oriT region
oriTDB accession number 200022
Name oriT_Tn5251  insolico
Sequence Completeness intact
oriT length 215 nt
oriT sequence AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)[61-76] [80-95] nt  (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT)
[123-134] [139-150] nt  (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site [135-136] nt
Conserved sequence flanking the
  nic site 		TGG|T
Note blastn alignment with the oriT_Tn916
L:61-76;R:80-95;L:123-134;R:139-150;D:135-136
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 236
Name Orf20_Tn5251 insolico
GenBank accession number ACW84401
Family MOBT
Length 401 aa
UniProt ID D3U1A0
PDB ID _
Pfam Rep_trans [PF02486.18], Evalue: 3.10E-69, Aligned region: 168..373
HTH_3 [PF01381.21], Evalue: 4.30E-10, Aligned region: 15..62
HTH_31 [PF13560.5], Evalue: 1.10E-06, Aligned region: 11..69
Note _
Protein sequence [Download] MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWE
ALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGD
IFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDK
TGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFV
DKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKL
DEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 325 _ insolico
Information of T4CP
ID 236
Name Orf21_Tn5251 insolico
SecReT4 accesion number _
GenBank accession number ACW84402
Family TrwB/TraD
Length 461
UniProt ID B7UTY5
PDB ID _
Pfam FtsK_SpoIIIE [PF01580.17], Evalue: 2.50E-07, Aligned region: 206..313
DUF87 [PF01935.16], Evalue: 4.40E-05, Aligned region: 216..352
Note FtsK-SpoIIIE family protein
Protein sequence [Download]

Reference
 N/A
Information of mobile element
ID 27
Element type _
ICE description Tn5251
ICEberg accesion number 47
GenBank accession number FJ711160.1
Family Tn916
Genome size 18033 bp
Coordinate of oriT  [+/-] 15378..15592 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus pneumoniae [1313]
Reference
[1] Santoro F et al (2010) Nucleotide sequence and functional analysis of the tet (M)-carrying conjugative transposon Tn5251 of Streptococcus pneumoniae. FEMS Microbiol Lett. 308(2):150-8. [PMID:20487027]
[2] Provvedi R1 et al (1996) Characterization of conjugative transposon Tn5251 of Streptococcus pneumoniae. FEMS Microbiol Lett. 135(2-3):231-6. [PMID:8595862]

Microbial Bioinformatics Group in MML, SJTU