oriTDB
The information of the oriT region
oriTDB accession number 200015
Name oriT_ICE_R88B  insolico
Sequence Completeness core
oriT length 40 nt
oriT sequence GAGGGAGGGCGCGCTTATACGTCGTGCCGACGTGCGTTTT
IR (inverted repeat)[19-24] [28-33] nt  (ACGTCG..CGACGT)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note _
L:19-24;R:28-33
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 229
Name TraA_ICE_R88B insolico
GenBank accession number ABM65826
Family MOBQ
Length 1016 aa
UniProt ID A2I7R8
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 1.00E-70, Aligned region: 17..216
AAA_30 [PF13604.5], Evalue: 1.10E-54, Aligned region: 351..540
AAA_19 [PF13245.5], Evalue: 1.20E-06, Aligned region: 356..483
Note conjugal transfer relaxase TraA
Protein sequence [Download] MAIYHLHVKVIGRKAGSSAVASAAYRSASRLRDERIERSHDFSAKRGVVHSEVMLPERAP
EAWRDRERLWNDVEAFEVRKDAQLAREVEFALPRELSKAQGIELARDFVQVEFVSKGMVA
DLNVHWDRAEDGSPKPHAHVMLTMRSVDKNGFGAKVRDWNATQLVERWRERWAELANERL
AELDIDARIDHRSLEAQGISLEPQTQIGAPAQRIEGGGLDAGEIEADRAELHREIARNNG
ARIIADPSVALDAITHQQSTFTRKDIAKFAHRHSDGMEQFNEVVVAISNASDLVELGTDL
RGEDRFTTRQMIQTEQRLHRATERMDLDERHAVSDAHREAALARAAQRGLVLSGEQTDAL
AHITDGHGLGVVVGFAGTGKSAMLGVARQAWAAAGYEVRGAALSGIAAENLEGGSGISSR
TIASMEHSWGQARDLLTTRDVLVIDEAGMVGTRQLERVLSHAADVGAKVVLVGDPQQLQA
IEAGAAFRSIHERHGGVEIGQVRRQREVWQRDATRDLATGRIGAAINAYDAQGMVHQAAT
RDDARRDLVERWDRDRQAHPEASRIILTHTNDEVRALNQAARERMRAAGDLGDEVQVTVE
RGPRNFASGDRVMFLRNERGLGVKNGTLGIVEEVSKQSMTVRTDDDRSVRFELKDYAYVD
HGYAATIHKAQGMTVDRTHVLATPGIDAHGSYVALSRHRDGMDLHYGSDDFSTRERLDRS
LSRDRAKDMASDYDQIDPAQSYAERRGITFRERVVEIVRRVVPEKLRDRIGGLLDVRSPG
DGEPMREGGRGPERENVGAQIGDARPMLVGENLAAGVSRETDTPVDAEAALRSARTKALV
RHARALDAVLSTGNADGQGSPDQMRELRDARSAFEKVRSYRWRDAEAAYMKNPELVREAG
AGRVSRIVLALQLETEIRTRMDIDPGRRADRFVERWQKLDRTGRQQYQAGDISGYKSARS
AMSDMAKSLERDPQLESLLANHKKALGIHVESGRRLGVELAFNHGIGLGRGKGIGI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 318 _ insolico
 N/A
Information of mobile element
ID 20
Element type _
ICE description ICE_R88B
ICEberg accesion number _
GenBank accession number EF157847.1
Family _
Genome size 37949 bp
Coordinate of oriT  [+/-] 20653..20692
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Mesorhizobium sp. R88B [418407]
Reference
[1] Carlton TM et al (2007) Ferrichrome utilization in a mesorhizobial population: microevolution of a three-locus system. Environ Microbiol. 9(12):2923-32. [PMID:17991023]