oriTDB

oriT
Relaxosome
T4CP
Element&Host

The information of the oriT region

oriTDB accession number	200010
Name	oriT_NBU1 (IME)
Sequence Completeness	intact
oriT length	214 nt
oriT sequence	TCCGCAAGGAATACAGCAGAGACCGATACATCCGTGACGCTTCACAGATTTACAGCGGAT GCAAGGACTTGAACGAATACTTACAGAAACAGGTTGAAAGAAAAAGGCAAGTCCAATCCG TCAAAGGGATGAGCAGCCAGTCACCGAAAAAGAAAAACGGCTTTCGGTTATAGCCCACTA TAACTACCTCCGCTTTCGTAGTTGTGGGCTCTCCGAGGGGA
IR (inverted repeat)	[143-149] [162-168] nt (ACCGAAA..TTTCGGT) [182-187] [198-203] nt (AACTAC..GTAGTT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:143-149;R:162-168;L:182-187;R:198-203

Visualization of oriT structure

Reference

[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]

[2] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]

[3] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]

I. Information of Relaxase

ID	224
Name	MobN1_NBU1
GenBank accession number	YP_133705
Family	MOB_P
Length	467 aa
UniProt ID	_
PDB ID	_
Pfam
Note	NBU1 mobilization protein
Protein sequence [Download]

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	312	_

N/A

Information of mobile element

ID	15
Element type	IME (Mobilizable transposon)
ICE description	NBU1
ICEberg accesion number	_
GenBank accession number	NC_006373.1
Family	_
Genome size	10276 bp
Coordinate of oriT [+/-]	8213..8426 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacteroides uniformis [820]

Reference

[1] Shoemaker NB et al (1988) Tetracycline-dependent appearance of plasmidlike forms in Bacteroides uniformis 0061 mediated by conjugal Bacteroides tetracycline resistance elements. J Bacteriol. 170(4):1651-7. [PMID:2832373]