oriTDB
The information of the oriT region
oriTDB accession number 200010
Name oriT_NBU1 (IME)  insolico
Sequence Completeness intact
oriT length 214 nt
oriT sequence TCCGCAAGGAATACAGCAGAGACCGATACATCCGTGACGCTTCACAGATTTACAGCGGAT GCAAGGACTTGAACGAATACTTACAGAAACAGGTTGAAAGAAAAAGGCAAGTCCAATCCG TCAAAGGGATGAGCAGCCAGTCACCGAAAAAGAAAAACGGCTTTCGGTTATAGCCCACTA TAACTACCTCCGCTTTCGTAGTTGTGGGCTCTCCGAGGGGA
IR (inverted repeat)[143-149] [162-168] nt  (ACCGAAA..TTTCGGT)
[182-187] [198-203] nt  (AACTAC..GTAGTT)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note _
L:143-149;R:162-168;L:182-187;R:198-203
Visualization of oriT structure
Reference
[1] Vedantam G et al (2006) Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation. Mol Microbiol. 59(1):288-300. [PMID:16359335]
[2] Wang J et al (2000) Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. J Bacteriol. 182(12):3559-71. [PMID:10852890]
[3] Li LY et al (1995) The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 177(14):3940-5. [PMID:7608064]
I. Information of Relaxase
ID 224
Name MobN1_NBU1 insolico
GenBank accession number YP_133705
Family MOBP
Length 467 aa
UniProt ID _
PDB ID _
Pfam
Note NBU1 mobilization protein
Protein sequence [Download]
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 312 _ insolico
 N/A
Information of mobile element
ID 15
Element type IME (Mobilizable transposon)
ICE description NBU1
ICEberg accesion number _
GenBank accession number NC_006373.1
Family _
Genome size 10276 bp
Coordinate of oriT  [+/-] 8213..8426 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bacteroides uniformis [820]
Reference
[1] Shoemaker NB et al (1988) Tetracycline-dependent appearance of plasmidlike forms in Bacteroides uniformis 0061 mediated by conjugal Bacteroides tetracycline resistance elements. J Bacteriol. 170(4):1651-7. [PMID:2832373]