oriTDB

The information of the oriT region

oriTDB accession number	100991
Name	oriT_pMKFS47-II
Sequence Completeness	core
oriT length	37 nt
oriT sequence	TACGCAGAAACGAAGTTACTGCGTATAAGTGCGCCCT
IR (inverted repeat)	[1-11] [15-25] nt (TACGCAGAAAC..GTTACTGCGTA)
Location of nic site	[32-33] nt
Conserved sequence flanking the nic site	TAAGTGC\|GCCCT
Note	predicted by the oriTfinder
	L:1-11;R:15-25;D:32-33

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TACGCAGAAACGAAGTTACTGCGTATAAGTGCGCCCT

Reference
N/A

I. Information of Relaxase

ID	1061
Name	MobA_pMKFS47-II
GenBank accession number	WP_047916715
Family	MOB_Q
Length	656 aa
UniProt ID	A0A0D6DZV3
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 3.10E-63, Aligned region: 18..242
Note	putative relaxase
Protein sequence [Download]	MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTSLYNHRLVQPEAFIMKPDFV PSEFSNREFLWNKMELSEKSANAQLCRELNVALPIELNKDDQKLLIKEFVKDNFISDGMI ADVAIHRDDENNPHAHIMLSMREVDSEGNILNKSCRIPKLDEEGNQVYNEKGHKVTVSVK TNNWNRKTFVSEIRTDWAKKVNHYLKERNIDQQITEKSHAELGKKELPSIHEGSYSNRLS SKGITSDLKLKNSDIQDFNAILSELDKLEKQEEILKNDKSFTLKFEKSFSPLEKKELKNL SKELKIFINDDNIEKRISELKRWENSLIFDNKMELQTQRIMLNKVNDEREMLLKANEVLD KQADRFFKKSYPILNTDKFSKSEIRAMVNETIFRKQLLNKDQLAEVIYNERVVEQEENKK IFKERPFQTSRYIDTKIKQIDESISSETNPQIKEVLSVKKEKLLGIKEGLIDYVQSEVSI KFDKNVSIDTVIEGEMLLAKSEYYKTTDFSKIEGVARFTSDEINSMLEQSKGYIGYIQSA KIPNDCQGVFFVQDSMKHLDKLSPLAKNNLKKIVNHNAYLPERDKVELSSKLEKTENIDT EEPEKPGMNVTMFQLAKSINRLVSGNVPQKKRNLDKLIKQTKAKENQTLQRNIPLR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1449
Plasmid name	pMKFS47-II
GenBank accession number	NZ_LN774770.1
Genome size	55671 bp
Coordinate of oriT [Strand]	33121..33157 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus piscium MKFS47 [297352]

Reference
N/A