oriTDB

The information of the oriT region

oriTDB accession number	100987
Name	oriT_pN3-7A-1
Sequence Completeness	intact
oriT length	106 nt
oriT sequence	CAGCCTCGCAGAGCAGGATTTCCGTTGAGCACCGCCAGGTGCGAATAAGGGCAAGTGAAG AAGGACAACCCGCTTGCGGGTGGGCCTACTTCACACATCCTGCCCG
IR (inverted repeat)	[55-73] [76-94] nt (GTGAAGAAGGACAACCCGC..GCGGGTGGGCCTACTTCAC)
Location of nic site	[102-103] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	predicted by the oriTfinder
	L:55-73;R:76-94;D:102-103

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CAGCCTCGCAGAGCAGGATTTCCGTTGAGCACCGCCAGGTGCGAATAAGGGCAAGTGAAG

61

AAGGACAACCCGCTTGCGGGTGGGCCTACTTCACACATCCTGCCCG

Reference
N/A

I. Information of Relaxase

ID	1057
Name	TrbI_pN3-7A-1
GenBank accession number	WP_089031315
Family	MOB_P
Length	598 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.30E-53, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIAKHIAMRSLGKSDFAGLVSYVTDAQSKDHRLGQVQITNCDAVSVQDAITEILATQHSN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA INKIHPARHTMHEPYYPHLALADLCTALEREYGLEPDNHQSHQRGAASRAADMERHAGVD SLVGWIKRECLEEIKAAQSWAELHQVMRDNSLEMTPRGNGLVIRADDGTMVKPSTVAREL SKPALERRLGAFEAPPEWVERKQAKREYQKKPVRSRADTTELYARYKDEQKNLTVARAEA LAAAKRRKDRAVAHAKQKAVTKRAAIKLAGGGKVSKKLLYSQTSATLRAELDGIHKAYAR EREWLFDGHQRRQWVDWLKHEATQGNSEALAALRAREAAQGLKGNTLAAQGEAKPGHAPV IDNITKKGTIIYRAGQSAVRDDGDRLQVSTGADRAGVLEALRLAAERYGDRITVNGTTEF KAQAIRAAVDGRLTITFADSALEKRRQALLQDNFSATRKPGVPGVGKAPPPAARAGRLRS LAQAEALQIEGAAAAVTPQRPAVALTPASSELARRKAKLQAELSAKKERTNQRKGRGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1445
Plasmid name	pN3-7A-1
GenBank accession number	NZ_CP022308.1
Genome size	427202 bp
Coordinate of oriT [Strand]	41083..41188 [-]
Drug resistance	_
Heavy-metal resistance	copper resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Alcanivorax sp. N3-7A [2014542]

Reference
N/A