oriTDB
The information of the oriT region
oriTDB accession number 100984
Name oriT_pABWA45_2  insolico
Sequence Completeness intact
oriT length 360 nt
oriT sequence GATTATCAAGAGTCACATGTGAATCGGCCATTTTTTTTATTAGAGTCAGTCTGGATTCAC CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC CTTTAATTTGTCTTAATTTAGTGGCCTGATTCGCAGATGAATCTGGATTCGACTCCTTTA CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCTGACATAGAACAACGATAA CAATATTAACATAATATACTGATAATAAAGGATAATCATGCAAGATTAAATCTTGCGACG GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACGGAAATATGTCAGCCAGGAAGAATTC
IR (inverted repeat)[280-287] [289-296] nt  (GCAAGATT..AATCTTGC)
Location of nic site [305-306] nt
Conserved sequence flanking the
  nic site 		GACGGGTGT|GG
Note predicted by the oriTfinder
L:280-287;R:289-296;D:305-306
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 1054
Name TraI_pABWA45_2 insolico
GenBank accession number WP_047719744
Family MOBF
Length 1765 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.20E-71, Aligned region: 7..280
TraI [PF07057.10], Evalue: 8.80E-46, Aligned region: 1439..1560
AAA_30 [PF13604.5], Evalue: 1.70E-35, Aligned region: 970..1162
AAA_19 [PF13245.5], Evalue: 7.80E-10, Aligned region: 975..1111
Note putative relaxase
Protein sequence [Download] MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG
SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMKEVEHLVSA
RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT
GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE
AAGPDASAKSRDVAALDTRQAKAWADPDLLKADWRRRLTDEKFDIGHYISQAQARVEITG
SVVAGQGGMRAPGQPGIGSSGEAADELVQKAVSDTISALSDKKVQFTWSEMLAGTVSRLP
SAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF
PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRDRTEDVAMMARSQGREVMVIAA
DGRSGQFLSESPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG
AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLISEADKRSRYGQLAQEYVR
LSAEGRDVVAQVTGTREQQQLTEVIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN
YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT
LEVSEGDRVRALGRELKGAIKAKEQFTLAGLENGAVRLRSGDRELRLPTERAVKLTHDYV
EGTGAGTSASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPLAPEQAARKVESVSAVRL
ASDQVRQSTGEANLDTAIQASRDRLMSDAEQAVSLAIPRAQQGQVHLSEITLLSEAVKSG
QPLADVRTEIARQVNSGELIQLDSVSGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLM
ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP
QVIGLGPTHRAVHEMREAGVDARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR
DMAELYQLVTAGGGRMVSSGDTAQLQAISTGQPFRLVQQRSAIDTVVMQEIVRQTPALRP
AIESIIAGQVDTSLRLVDDVSPQQVPRQEGAWVPGNSVMEIRAPKKDQEQDTPAADEQTL
TPEQLSLVRTDIIEAIRDDWMGRTPEAQLQTLVVAELNADRHAINDAIHAARHEKGDTGA
EERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGES
VLISPQQNTAQDISLFTPRDLTISHGDRVRFTRSDNDRGYVANSLWEVAGFTDDGAIRFR
QGEQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVT
LSRAKEHVQVYTDDLAGWSAGARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDKTA
LGRRVLAENGLEGETMARFIAAGRKYPTPYVALPAWTRHGQEAGALLTEIRIEDDGMRVV
LSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLLNA
ARLTGGRITEPDEVARTVRSVAEVESAAKAEDPITLPPDEQQKLAEAQEKAARELAEQAR
QELLPAVPGEESTRPEPLLSADEERRLRDGTARGERELDEAIQEAVAEGRGIRQQVREQM
LRTEREWVVNVPEKDIELEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1442
Plasmid name pABWA45_2
GenBank accession number NZ_CP022156.1
Incompatibility group IncFII(SARC14)
Genome size 113793 bp
Coordinate of oriT  [Strand] 79709..80068 [-]
Drug resistance _
Heavy-metal resistance insolico mercury resistance; copper resistance; arsenical resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli ABWA45 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU