oriTDB

The information of the oriT region

oriTDB accession number	100970
Name	oriT_pM270-accessoryB
Sequence Completeness	core
oriT length	50 nt
oriT sequence	TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTGCTCAGAGGGCC
IR (inverted repeat)	[19-24] [29-34] nt (CGTCGC..GCGACG)
Location of nic site	[8-9] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:19-24;R:29-34;D:8-9

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTGCTCAGAGGGCC

Reference
N/A

I. Information of Relaxase

ID	1040
Name	TraA_pM270-accessoryB
GenBank accession number	WP_088199054
Family	MOB_Q
Length	1100 aa
UniProt ID	_
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 3.60E-75, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 3.70E-55, Aligned region: 381..569 AAA_19 [PF13245.5], Evalue: 5.60E-10, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 0.00016, Aligned region: 656..737
Note	putative relaxase
Protein sequence [Download]	MAIAHFSASIVSRGGGRSVVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPGDAP KWVKALIADRLVSGAVEAFWNKVEAFEKRADAQLARDLTIALPLELTPEQNIALVRDFAE KHILTKGMVADWVYHDNPGNPHIHLMTTLRPLTGDGFGSKKVAVIGEDGQPVRTKSGKIL YQLWAGSTDDFNALRDGWFERLNHHLALGGIDLRIDGRSYEKQGIDLEPTIHLGVGAKAI ERKAQEQGVRPELERIELNEQRRSENTRRILNNPAIVLDLITREKSVFDERDVAKVLHRY VDDPTVFQQVMLRIILNPEVLRLQRDTIEFATGEKVPACYSTRAMIRLEATMVRQALWLS NRDGHAVSEAALDATFRRHERLSQEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREA WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWNRARDVLDDKTVFVMDEAGMV ASRQMAGFVDAVVRAGAKIVVVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWM RKASLDLARGNVEKALTAYDANVRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRR DVRMLNVMARKKLVERGIVGEGHVFKTADGIRQFDAGDQIVFLKNETSLGVKNGMIAHVI EAAPNRIVAVVGEGDQRRQVIVEQRFYSNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDVQLYYGRRSFAFNGGLAKVLSRRNAKETTLDYERGKLHREALRFAESR GLNIVQVARTLVRDRLDWTLRQKMKLVELGQRLAVFAERLGLHHPLKTQTMKEAAPMVAG IKTFSGSVADTVEQKLDTDPALRKQWQEVSARFAYVFADPETAFRAMNFDAVLTEKDTAR QVVQKLEAEPASIGPLRGKTGILASKAERESRRIAEINVPALKRDLEQYLRMREAAIKRL STDEQVQRQRVSIDIPALSPAAHVVLERVRDAIDRNDLPAALGYALSNRETKLEIDGFNK AVTERFGERTLLTNAARQPSGTLFDKLSNGMQPEQKERLKEAWPVMRTAQQIAAHERTTQ TLKQAEELRLTQRQSPVMKQ

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1428
Plasmid name	pM270-accessoryB
GenBank accession number	NZ_CP021816.1
Genome size	203845 bp
Coordinate of oriT [Strand]	36143..36192 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sinorhizobium meliloti M270 [382]

Reference
N/A