oriTDB
The information of the oriT region
oriTDB accession number 100956
Name oriT_p2474-3  insolico
Sequence Completeness intact
oriT length 110 nt
oriT sequence TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [24-31] [35-42] nt  (GTCGGGGC..GCCCTGAC)
IR2: [49-65] [72-88] nt  (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site [96-97] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:24-31;R:35-42;L:49-65;R:72-88;D:96-97
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 1026
Name Mob_p2474-3 insolico
GenBank accession number WP_086353228
Family MOBQ
Length 1210 aa
UniProt ID _
PDB ID _
Pfam
Note putative relaxase
Protein sequence [Download] MNESAEPSPSPDAVKEEMTVMTEPDDKQSIGISPDTPREEAVNHVTEDIPLQPEPFLPES
DGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIEPAVRDE
PTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTPPIPDSIVY
APERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMVNGASNDDRSV
LAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAELEKLRKEMAVSK
DAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTVSTAPASSPAEPGKT
ADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELKNRSGSKLYWGEQLESL
VKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNGISVSHDNPDKGQRIQAIP
VETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNANQVTEITSAAPDK
LTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLYPALATPSADNSQLVINALTDKG
LRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPLFAAVYTPEKQPDALYRNLGFEQSW
QQWSNSQKPEDRQEKTLHQDLSHSPGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1414
Plasmid name p2474-3
GenBank accession number NZ_CP021208.1
Incompatibility group IncI1
Genome size 86725 bp
Coordinate of oriT  [Strand] 58494..58603 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli strain Z247 [562]
Reference
[1] Zheng B et al (2016) Coexistence of MCR-1 and NDM-1 in Clinical Escherichia coli Isolates. Clin Infect Dis. 63(10):1393-1395. [PMID:27506685]

Microbial Bioinformatics Group in MML, SJTU