oriTDB

The information of the oriT region

oriTDB accession number	100955
Name	oriT_p1002-MCR1
Sequence Completeness	core
oriT length	53 nt
oriT sequence	CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
IR (inverted repeat)	[5-21] [25-42] nt (CGATTGTAACATGACCG..CGGTCTTGTGTACAATCG)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	TATCGTG\|C
Note	predicted by the oriTfinder
	L:5-21;R:25-42;D:50-51

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT

Reference
N/A

I. Information of Relaxase

ID	1025
Name	Putative relaxase of p1002-MCR1
GenBank accession number	WP_034169409
Family	MOB_H
Length	990 aa
UniProt ID	A0A0R6LVD4
PDB ID	_
Pfam
Note	putative relaxase
Protein sequence [Download]	MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIIDSIENKSQERYL HITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHLPKIRHIQDNSTGELVE RKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQEFINNKYNLDSPKDYPRKDAD YGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSVFKDIVAEYGELRIRNAGKTNEY LAVKLPGDKKYINLKSPLFRQNYIETRTLTLEKPTHKEIEKRLNTWLNKTSQEIKHIFNQ AEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQTSGRAGGYKSCPKKFARIRQ SEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDLAEQLSQREHPGQDVRRDYDRQ FTESGIKSLERSSFLCETMFQTLNEAAEKNEIATMAEIRRNIDPVRFLSSAAERFNIIPA QHKIRTAKDGSPRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPT YRRLTHHEARERLNSLNLSEKTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVI VYKKLTTLERLSELDTEGRHIIRQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPE LSLQKAVDSQRRLEEAKKVNSKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNP SKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAE LQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQATEQAQATEQAQATEQA QATEQEQATEQEQVTEQEQATEQEQATEQAQATEQAQATEQAQVAAQATSSYDPGVITRA NTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFDVASFDNENDAKEFCK IVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDLNEGIVFHSKDAAIQ CYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEGALVTFDRPFEIIANSY EEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHGLNQDDILITPVTREEIAF KGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCNGD IVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSA RVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELH DLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFED KFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREEHLELQQQRDNSQDMH

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1413
Plasmid name	p1002-MCR1
GenBank accession number	NZ_CP021205.1
Incompatibility group	IncI2
Genome size	63392 bp
Coordinate of oriT [Strand]	28384..28436 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Z1002 [562]

Reference

[1] Zheng B et al (2016) Coexistence of MCR-1 and NDM-1 in Clinical Escherichia coli Isolates. Clin Infect Dis. 63(10):1393-1395. [PMID:27506685]