oriTDB

The information of the oriT region

oriTDB accession number	100954
Name	oriT_p1002-1
Sequence Completeness	intact
oriT length	550 nt
oriT sequence	TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCG AAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGA GAGCGATATC
IR (inverted repeat)	[243-255] [257-269] nt (TATTTAATAATTC..GAATTATTAAATA) [311-318] [321-328] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[337-338] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:243-255;R:257-269;L:311-318;R:321-328;D:337-338

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	1024
Name	Relaxase_p1002-1
GenBank accession number	WP_000130000
Family	MOB_C
Length	101 aa
UniProt ID	Q0ZKT4
PDB ID	_
Pfam	PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85 HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94
Note	putative relaxase
Protein sequence [Download]	MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1412
Plasmid name	p1002-1
GenBank accession number	NZ_CP021203.1
Incompatibility group	p0111
Genome size	183508 bp
Coordinate of oriT [Strand]	137885..138434 [-]
Drug resistance	tellurite/colicin resistance; tunicamycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Z1002 [562]

Reference

[1] Zheng B et al (2016) Coexistence of MCR-1 and NDM-1 in Clinical Escherichia coli Isolates. Clin Infect Dis. 63(10):1393-1395. [PMID:27506685]