oriTDB

The information of the oriT region

oriTDB accession number	100948
Name	oriT_pVISLISI_2
Sequence Completeness	intact
oriT length	239 nt
oriT sequence	AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATT ATCCCCAACAAGCAGGCGACGCTACCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA CCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAATGTAAGAAAGGCGTT ATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC
IR (inverted repeat)	IR1: [52-59] [61-68] nt (TTGGGGAT..ATCCCCAA) IR2: [94-98] [104-108] nt (TGGCT..AGCCA)
Location of nic site	[115-116] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	predicted by the oriTfinder
	L:52-59;R:61-68;L:94-98;R:104-108;D:115-116

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATT

61

ATCCCCAACAAGCAGGCGACGCTACCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

121

CCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAATGTAAGAAAGGCGTT

181

ATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC

Reference
N/A

I. Information of Relaxase

ID	1018
Name	Rlx_pVISLISI_2
GenBank accession number	WP_084944766
Family	MOB_P
Length	336 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.10E-82, Aligned region: 8..242
Note	putative relaxase
Protein sequence [Download]	MATTKISATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKATRELYDKTDGNQGHVIIQ SFKPGEVTPEQCNQLGLELAEKLAPNHQVAVYTHNDTDHVHNHIVINSIDLETGLKFNNN KQALRNVRDFNDEVCMKHGLSVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDKIREAI DQSQATNMDEFKDHLNQHGIEIERVTPKSITYRHLAEDKKVRGRKLGEDYNKGGIEDGFE RQIQRRRQQERDSDTECQPKRPTSSRDESTERDTGVTQSDWDQFAQDTNELERSRQAAES ARLADEKARRDREERERAKQASRRIINNDRDHGLEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1406
Plasmid name	pVISLISI_2
GenBank accession number	NZ_CP020736.1
Genome size	6453 bp
Coordinate of oriT [Strand]	3648..3886 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus lugdunensis VISLISI_27 [28035]

Reference
N/A