oriTDB

The information of the oriT region

oriTDB accession number	100946
Name	oriT_pLd2
Sequence Completeness	intact
oriT length	417 nt
oriT sequence	CCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGACGAAAATCGTCTCTTTTTTTCTT CTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTT GGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTT CCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAG CTTGCAGTATTTCTGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGG CGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATT TTATCTTTGAGGAGGCTATTTATGGATCAAAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [161-173] [177-189] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR2: [195-209] [222-236] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[244-245] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:161-173;R:177-189;L:195-209;R:222-236;D:244-245

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGACGAAAATCGTCTCTTTTTTTCTT

61

CTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTT

121

GGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTT

181

CCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAG

241

CTTGCAGTATTTCTGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGG

301

CGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATT

361

TTATCTTTGAGGAGGCTATTTATGGATCAAAAAGAAGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	1016
Name	Relaxase_pLd2
GenBank accession number	WP_086383686
Family	MOB_P
Length	410 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.40E-75, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1404
Plasmid name	pLd2
GenBank accession number	NZ_CP020606.1
Genome size	15218 bp
Coordinate of oriT [Strand]	9180..9596 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis FM03 [44688]

Reference
N/A