oriTDB
The information of the oriT region
oriTDB accession number 100938
Name oriT_pGeo12a  insolico
Sequence Completeness core
oriT length 34 nt
oriT sequence GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)[4-18] [22-34] nt  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site [2-3]nt
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L:4-18;R:22-34;D:2-3
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 1008
Name Nickase_pGeo12a insolico
GenBank accession number WP_087960389
Family MOBQ
Length 653 aa
UniProt ID _
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 3.20E-66, Aligned region: 16..233
Note putative relaxase
Protein sequence [Download] MAYFMFRTQIINKADRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPE
WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD
IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD
RINEKFKEKDRINEKFKEKGINDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEK
QGKPYEPVTYYGKLNQEIQRINQELKALKQQEKVVSISEYQEEKSLNETIESIRKNASLN
DVEKASLLLVAKRAKSYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVY
QQEPKQVIQYGFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLT
EEEFRVLYPHAGADFRIEEKHHAVQYFKDTGGVLPESEIPAYAAKKEQEIHRMPTFAEQT
RNMYQSLFVLNRTIQKQSKERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAV
LQAILSEHYGRDVSITNTAALLRLHELVERGESSKDFEADLRRIHVSEQQKLERMPKPET
EAQIMEYQYRQHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1396
Plasmid name pGeo12a
GenBank accession number NZ_CP020031.1
Genome size 58808 bp
Coordinate of oriT  [Strand] 640..673 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Geobacillus thermodenitrificans T12 [33940]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU