oriTDB

The information of the oriT region

oriTDB accession number	100936
Name	oriT_pASM4
Sequence Completeness	intact
oriT length	174 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCACGGATGCGAGTGTCAGTGAAATGCTTCATGTGGCAGGAGAAAAATGCAGC AGCGGAGCGTCAGCAGAATATGTGATACAAGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC

61

TATGTTGGCACGGATGCGAGTGTCAGTGAAATGCTTCATGTGGCAGGAGAAAAATGCAGC

121

AGCGGAGCGTCAGCAGAATATGTGATACAAGATATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	1006
Name	MobA_pASM4
GenBank accession number	WP_012565229
Family	MOB_P
Length	499 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-16, Aligned region: 49..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSF AEADLPSGQREKLMASFERVLMPGLDKDQYSILWVEHRDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRHPRPRPETVLSHEPAHERHG AHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQ KALELERSQRQRTYNSPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1394
Plasmid name	pASM4
GenBank accession number	NZ_CP019843.1
Genome size	4667 bp
Coordinate of oriT [Strand]	679..852 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae R11 [550]

Reference
N/A