oriTDB

The information of the oriT region

oriTDB accession number	100926
Name	oriT_pCFSAN001297_01
Sequence Completeness	intact
oriT length	355 nt
oriT sequence	ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT AGCATATATGCGGATTAATTCCATATGTGTAATACGTTGCGTATTACACATCAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTAAGTG CAAGTGAATCGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG
IR (inverted repeat)	IR1: [145-155] [161-171] nt (ATGTGTAATAC..GTATTACACAT) IR2: [208-218] [223-233] nt (GCATTTACGAT..ATCGTAATTGC) IR3: [336-344] [347-355] nt (CTTTCATGC..GCATGAAAG)
Location of nic site	[242-243] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	predicted by the oriTfinder
	L:145-155;R:161-171;L:208-218;R:223-233;L:336-344;R:347-355;D:242-243

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC

61

TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT

121

AGCATATATGCGGATTAATTCCATATGTGTAATACGTTGCGTATTACACATCAAAACACA

181

AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT

241

GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTAAGTG

301

CAAGTGAATCGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG

Reference
N/A

I. Information of Relaxase

ID	997
Name	Relaxase_pCFSAN001297_01
GenBank accession number	WP_023223526
Family	MOB_F
Length	969 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 3.60E-79, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659 Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..654 Viral_helicase1 [PF01443.17], Evalue: 2.30E-06, Aligned region: 780..897 UvrD_C_2 [PF13538.5], Evalue: 2.10E-09, Aligned region: 849..896
Note	putative relaxase
Protein sequence [Download]	MLNITTITRQRVDKVVDYYTDGADDYYAKDGNAMQWQGAGAEELGLTGEVEQQRFAKLLD GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVSGDKRIIEAHDRAVAKAIEE AERLALGRFTVNKKTHVENTNKLIVAKFRHETSRELDPELHTHAFVMNLTKTSDGKWRSL TNDGIVNSLTLLGNVYKSELARDLELAGYNLRYDRNGTFDLAHFTPEQTAHFSQRSQQIE AELAKDGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQAELGLKGQGINYPLLNRLDT TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE KKLDKAASREGFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKN NDERAFTSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1385
Plasmid name	pCFSAN001297_01
GenBank accession number	NZ_CP019200.1
Genome size	34175 bp
Coordinate of oriT [Strand]	12523..12877 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica 315 [1124958]

Reference

[1] Timme RE et al (2013) Phylogenetic diversity of the enteric pathogen Salmonella enterica subsp enterica inferred from genome-wide reference-free SNP characters. Genome Biol Evol. 5(11):2109-23. [PMID:24158624]