oriTDB

The information of the oriT region

oriTDB accession number	100911
Name	oriT_pEC542_KPC
Sequence Completeness	intact
oriT length	309 nt
oriT sequence	CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC GGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACG CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA GTTAGCACG
IR (inverted repeat)	IR1:[79-85] [89-95] nt (CAAGGGGC..GCCCCTTG) IR2: [153-160] [164-171] nt (CACGTGGC..GCCATGTG)
Location of nic site	[190-191] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:79-85;R:89-95;L:153-160;R:164-171;D:190-191

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG

61

CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC

121

GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC

181

GGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACG

241

CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA

301

GTTAGCACG

Reference
N/A

I. Information of Relaxase

ID	982
Name	Mob_pEC542_KPC
GenBank accession number	WP_033996285
Family	MOB_P
Length	890 aa
UniProt ID	A0A1J8JDJ9
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 4.00E-70, Aligned region: 548..807 Relaxase [PF03432.13], Evalue: 3.40E-30, Aligned region: 16..274
Note	putative relaxase
Protein sequence [Download]	MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIA LSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLH LHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREH IDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKT GSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKD YITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMRSVI AAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAA LQLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKS EQLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLR LQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITVPKL GTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLLKAE RRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRDVIK RQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1370
Plasmid name	pEC542_KPC
GenBank accession number	NZ_CP018968.1
Incompatibility group	IncP(6)
Genome size	44320 bp
Coordinate of oriT [Strand]	30126..30434 [-]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Ecol_542 [562]

Reference
N/A