oriTDB

The information of the oriT region

oriTDB accession number	100904
Name	oriT_pEC224_5
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

ACCATGCGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	975
Name	Putative relaxase of pEC224_5
GenBank accession number	WP_024623332
Family	MOB_P
Length	517 aa
UniProt ID	A0A1U9SBD2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSISPVYREWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1363
Plasmid name	pEC224_5
GenBank accession number	NZ_CP018943.1
Incompatibility group	ColRNAI
Genome size	6647 bp
Coordinate of oriT [Strand]	5449..5531 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Ecol_224 [562]

Reference
N/A