oriTDB

The information of the oriT region

oriTDB accession number	100901
Name	oriT_pKp_Goe_641-4
Sequence Completeness	intact
oriT length	181 nt
oriT sequence	TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTAAATATGTTACGGCGGAGTGGTAATGTATGAAAGTGCTTCATGTGGGAAGGGGAAAA TGTGGCTGACGGGCGTAAGTGCAACATGCTGGTAAAGGTGAAATGACGCCTCCTCGCTCA C
IR (inverted repeat)	[10-17] [21-28] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTAA
Note	predicted by the oriTfinder
	L:10-17;R:21-28;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG

61

GCTAAATATGTTACGGCGGAGTGGTAATGTATGAAAGTGCTTCATGTGGGAAGGGGAAAA

121

TGTGGCTGACGGGCGTAAGTGCAACATGCTGGTAAAGGTGAAATGACGCCTCCTCGCTCA

181

C

Reference
N/A

I. Information of Relaxase

ID	972
Name	Nuclease_pKp_Goe_641-4
GenBank accession number	WP_071890506
Family	MOB_P
Length	502 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.90E-16, Aligned region: 41..278
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSF AEAELLPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRL QPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLA LASSGELKTREDVTGALTAAGFEVVRTTKNSISIADPDGGRNIRLKGAIYEQSFSAGEGL GAAIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRHQRPRAGYDRGDAVEPPERA AYGRPDVADHHPGLGTADRHEREHSVVAGAADHRELPDYPGAGADAGHVVGEKPRRSAVA LRGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGTGKTVADRIRAATSGLLAKAGRVGE RLRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQR QHEKELAEARSRQRSYNGPTLG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1360
Plasmid name	pKp_Goe_641-4
GenBank accession number	NZ_CP018740.1
Genome size	4052 bp
Coordinate of oriT [Strand]	3860..4040 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae Kp_Goe_121641 [573]

Reference
N/A