oriTDB

The information of the oriT region

oriTDB accession number	100883
Name	oriT_pCAV1752-3851
Sequence Completeness	intact
oriT length	186 nt
oriT sequence	GGTTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTA TACTGGCTTAATATGATAAATCGAAGTGGTAATTCGGGAAAGTGCTTCATGTGGCAGGGG AAAAATTCAGCTGCGGGGCGTAAGTGCAAAATGCAGGTGGTGATGACATGACGCCTCCTC GCTCAC
IR (inverted repeat)	[15-22] [26-33] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[66-67] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:15-22;R:26-33;D:66-67

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTA

61

TACTGGCTTAATATGATAAATCGAAGTGGTAATTCGGGAAAGTGCTTCATGTGGCAGGGG

121

AAAAATTCAGCTGCGGGGCGTAAGTGCAAAATGCAGGTGGTGATGACATGACGCCTCCTC

181

GCTCAC

Reference
N/A

I. Information of Relaxase

ID	954
Name	Nuclease_pCAV1752-3851
GenBank accession number	WP_074184816
Family	MOB_P
Length	508 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.20E-15, Aligned region: 49..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEIRELIDASPYVKKYTSGVLSF AEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGKRL QPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGLPETKQEAAETITRGLLM LASSGELKTRDDVTGALTAAGFEVVRTTKNSISIADPDGGRNIRLRGAIYEQSFNGLEGL RGEIESTAAAYRREAESRIQQARERCQIGTERKREENQRRHQRPRTGDERGDAVEPAERA ANGRADVADHHHGVRAAVRQPERGAVVPGEPDSGEPGGNQAAERYAVETADADVGRDLSG KPQRTVSGAAGGDESRNGPEGGQQDAERGEAGTGVEQNDGTGKTIAERVRAATAGLLEKA GRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEFERAAAPVIDRLNEIEQQRYGQT TEKEPEKTADLVPERQRTLSHGDREYGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1342
Plasmid name	pCAV1752-3851
GenBank accession number	NZ_CP018357.1
Genome size	3851 bp
Coordinate of oriT [Strand]	3018..3203 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella oxytoca CAV1752 [571]

Reference
N/A