oriTDB

The information of the oriT region

oriTDB accession number	100877
Name	oriT_pHSL2
Sequence Completeness	intact
oriT length	190 nt
oriT sequence	GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATGGGATTGAGCGTAACGGTTAA CATTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAG CGGTGGCAACTGCCACCGCTCTAAATTCCACGTCGCCTCGATGCTGAGTGAACGTGGACG GTAAAAGCTA
IR (inverted repeat)	IR1: [19-38] [39-58] nt (AACCGTGAAGCGCGATCCGA..TGGGATTGAGCGTAACGGTT) IR2: [117-128] [131-142] nt (AAAGCGGTGGCA..TGCCACCGCTCT)
Location of nic site	[86-87] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:19-38;R:39-58;L:117-128;R:131-142;D:86-87

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATGGGATTGAGCGTAACGGTTAA

61

CATTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAG

121

CGGTGGCAACTGCCACCGCTCTAAATTCCACGTCGCCTCGATGCTGAGTGAACGTGGACG

181

GTAAAAGCTA

Reference
N/A

I. Information of Relaxase

ID	948
Name	VirD2_pHSL2
GenBank accession number	WP_056379678
Family	MOB_P
Length	347 aa
UniProt ID	A0A1L4A0K2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.70E-06, Aligned region: 72..266
Note	putative relaxase
Protein sequence [Download]	MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARVVSRAP EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAELARDWGDPVYWRD NSTVAAVSMVFSMPSGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR GDTGRRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA GTAPAPFANQKITAAARDQLAGRSTAPDFVVRGRQAWNETHRRYLAAAKRLEVSSDPADR QLADQVREFVGAGRTPTIHERSVAAIERKRQNERSRDRDRSREGPER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1336
Plasmid name	pHSL2
GenBank accession number	NZ_CP018223.1
Genome size	61559 bp
Coordinate of oriT [Strand]	27601..27790 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sphingomonas sp. JJ-A5 [1921510]

Reference
N/A