oriTDB
The information of the oriT region
oriTDB accession number 100871
Name oriT_pMRSN346595_62.2  insolico
Sequence Completeness intact
oriT length 470 nt
oriT sequence AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATAAAATTTGTCAGTACTGACCCTAAC AAGGGTCGCCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATTAAAAACATAAGCTAATGATTCAAAAAGCAAATCAGCAAAAACTT GTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACTAGTTGAGCCTTATA GTGGAGTAGGTTAAATTATTTCCGGATAATGTCACCAGAGGTGAAAAAATGAAAAAGTGG ATGTTAGCAATCTTCCTGATGTTTATAAGTGGGATCTGTGAAGCCGCCGATTGTTTTGAT CTTGCAGGCCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC
IR (inverted repeat)[142-151] [154-163] nt  (ATAGTGTCGT..ACGACCCTAT)
[231-238] [241-248] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [257-258] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:142-151;R:154-163;L:231-238;R:241-248;D:257-258
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 942
Name TraI_pMRSN346595_62.2 insolico
GenBank accession number WP_071940847
Family MOBF
Length 1756 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.20E-76, Aligned region: 10..284
TraI [PF07057.10], Evalue: 4.30E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.00E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 1.00E-08, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV
GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRAE
IRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRGAQGETQVVRISSLDSSWSLFRPEKMPVAGGERLRV
TGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPATLPVSNSPFTALKLESGWVETPGH
SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTS
FVDLGREINAQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYTLIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQEAMQKGEA
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVSLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
RVIRPAQEQAEQHIDLAYAITAHGAQGASETFAIVLEGTEGNRKLMAGFESAYVALSRMK
QHVQVYTDDRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQME
RDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1330
Plasmid name pMRSN346595_62.2
GenBank accession number NZ_CP018113.1
Incompatibility group IncFII(29)
Genome size 62157 bp
Coordinate of oriT  [Strand] 24436..24905 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli MRSN346595 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU