oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100865
Name	oriT_pCAV1016-76
Sequence Completeness	core
oriT length	105 nt
oriT sequence	AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACG TCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
IR (inverted repeat)	[18-35] [42-59] nt (GTTTTTGGTACACCGCCG..CGGCAGTGACGCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:18-35;R:42-59

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	937
Name	Putative relaxase of pCAV1016-76
GenBank accession number	WP_004206924
Family	MOB_P
Length	659 aa
UniProt ID	A0A0U3I0I2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.70E-30, Aligned region: 13..334
Note	putative relaxase
Protein sequence [Download]	MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTR KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSW PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS FTKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKE GVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFAGIGLRVEKAQGKKGYVI THEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDA NKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCN RIVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQLRSESDDKFNPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1325
Plasmid name	pCAV1016-76
GenBank accession number	NZ_CP017936.1
Incompatibility group	IncL/M(pMU407)
Genome size	76186 bp
Coordinate of oriT [Strand]	11218..11322 [+]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae CAV1016 [573]

Reference

[1] Mathers AJ et al (2015) Klebsiella pneumoniae carbapenemase (KPC)-producing K pneumoniae at a single institution: insights into endemicity from whole-genome sequencing. Antimicrob Agents Chemother. 59(3):1656-63. [PMID:25561339]