 oriTDB accession number | 100863 |
| Name | oriT_pKPC_UVA02  |
Sequence Completeness  | intact |
| oriT length | 360 nt |
| oriT sequence | GATTATCAAGAGTCACATGTGAATCGGCCATTTTTTTTATTAGAGTCAGTCTGGATTCAC CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC CTTTAATTTGTCTTAATTTAGTGGCCTGATTCGCAGATGAATCTGGATTCGACTCCTTTA CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCTGACATAGAACAACGATAA CAATATTAACATAATATACTGATAATAAAGGATAATCATGCAAGATTAAATCTTGCGACG GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACGGAAATATGTCAGCCAGGAAGAATTC |
| IR (inverted repeat) | [280-287] [289-296] nt (GCAAGATT..AATCTTGC) |
| Location of nic site | [305-306] nt |
| Conserved sequence flanking the nic site | GACGGGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 935 |
| Name | TraI_pKPC_UVA02 |
| GenBank accession number | WP_047719744 |
| Family | MOBF |
| Length | 1765 aa |
| UniProt ID | _ |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.20E-71, Aligned region: 7..280 TraI [PF07057.10], Evalue: 8.80E-46, Aligned region: 1439..1560 AAA_30 [PF13604.5], Evalue: 1.70E-35, Aligned region: 970..1162 AAA_19 [PF13245.5], Evalue: 7.80E-10, Aligned region: 975..1111 |
| Note | putative relaxase |
| Protein sequence [Download] | MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMKEVEHLVSA RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE AAGPDASAKSRDVAALDTRQAKAWADPDLLKADWRRRLTDEKFDIGHYISQAQARVEITG SVVAGQGGMRAPGQPGIGSSGEAADELVQKAVSDTISALSDKKVQFTWSEMLAGTVSRLP SAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRDRTEDVAMMARSQGREVMVIAA DGRSGQFLSESPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLISEADKRSRYGQLAQEYVR LSAEGRDVVAQVTGTREQQQLTEVIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT LEVSEGDRVRALGRELKGAIKAKEQFTLAGLENGAVRLRSGDRELRLPTERAVKLTHDYV EGTGAGTSASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPLAPEQAARKVESVSAVRL ASDQVRQSTGEANLDTAIQASRDRLMSDAEQAVSLAIPRAQQGQVHLSEITLLSEAVKSG QPLADVRTEIARQVNSGELIQLDSVSGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLM ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP QVIGLGPTHRAVHEMREAGVDARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR DMAELYQLVTAGGGRMVSSGDTAQLQAISTGQPFRLVQQRSAIDTVVMQEIVRQTPALRP AIESIIAGQVDTSLRLVDDVSPQQVPRQEGAWVPGNSVMEIRAPKKDQEQDTPAADEQTL TPEQLSLVRTDIIEAIRDDWMGRTPEAQLQTLVVAELNADRHAINDAIHAARHEKGDTGA EERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGES VLISPQQNTAQDISLFTPRDLTISHGDRVRFTRSDNDRGYVANSLWEVAGFTDDGAIRFR QGEQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVT LSRAKEHVQVYTDDLAGWSAGARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDKTA LGRRVLAENGLEGETMARFIAAGRKYPTPYVALPAWTRHGQEAGALLTEIRIEDDGMRVV LSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLLNA ARLTGGRITEPDEVARTVRSVAEVESAAKAEDPITLPPDEQQKLAEAQEKAARELAEQAR QELLPAVPGEESTRPEPLLSADEERRLRDGTARGERELDEAIQEAVAEGRGIRQQVREQM LRTEREWVVNVPEKDIELEKTLGGD |
Reference| ID | 1323 |
| Plasmid name | pKPC_UVA02 |
| GenBank accession number | NZ_CP017929.1 |
| Incompatibility group | IncFII(SARC14) |
| Genome size | 113105 bp |
| Coordinate of oriT [Strand] | 28729..29088 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | copper resistance; arsenical resistance; mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Klebsiella oxytoca CAV1015 [571] |
Reference| [1] Mathers AJ et al (2015) Klebsiella pneumoniae carbapenemase (KPC)-producing K pneumoniae at a single institution: insights into endemicity from whole-genome sequencing. Antimicrob Agents Chemother. 59(3):1656-63. [PMID:25561339] |