oriTDB

The information of the oriT region

oriTDB accession number	100863
Name	oriT_pKPC_UVA02
Sequence Completeness	intact
oriT length	360 nt
oriT sequence	GATTATCAAGAGTCACATGTGAATCGGCCATTTTTTTTATTAGAGTCAGTCTGGATTCAC CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC CTTTAATTTGTCTTAATTTAGTGGCCTGATTCGCAGATGAATCTGGATTCGACTCCTTTA CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCTGACATAGAACAACGATAA CAATATTAACATAATATACTGATAATAAAGGATAATCATGCAAGATTAAATCTTGCGACG GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACGGAAATATGTCAGCCAGGAAGAATTC
IR (inverted repeat)	[280-287] [289-296] nt (GCAAGATT..AATCTTGC)
Location of nic site	[305-306] nt
Conserved sequence flanking the nic site	GACGGGTGT\|GG
Note	predicted by the oriTfinder
	L:280-287;R:289-296;D:305-306

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATTATCAAGAGTCACATGTGAATCGGCCATTTTTTTTATTAGAGTCAGTCTGGATTCAC

61

CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC

121

CTTTAATTTGTCTTAATTTAGTGGCCTGATTCGCAGATGAATCTGGATTCGACTCCTTTA

181

CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCTGACATAGAACAACGATAA

241

CAATATTAACATAATATACTGATAATAAAGGATAATCATGCAAGATTAAATCTTGCGACG

301

GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACGGAAATATGTCAGCCAGGAAGAATTC

Reference
N/A

I. Information of Relaxase

ID	935
Name	TraI_pKPC_UVA02
GenBank accession number	WP_047719744
Family	MOB_F
Length	1765 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 2.20E-71, Aligned region: 7..280 TraI [PF07057.10], Evalue: 8.80E-46, Aligned region: 1439..1560 AAA_30 [PF13604.5], Evalue: 1.70E-35, Aligned region: 970..1162 AAA_19 [PF13245.5], Evalue: 7.80E-10, Aligned region: 975..1111
Note	putative relaxase
Protein sequence [Download]	MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMKEVEHLVSA RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE AAGPDASAKSRDVAALDTRQAKAWADPDLLKADWRRRLTDEKFDIGHYISQAQARVEITG SVVAGQGGMRAPGQPGIGSSGEAADELVQKAVSDTISALSDKKVQFTWSEMLAGTVSRLP SAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRDRTEDVAMMARSQGREVMVIAA DGRSGQFLSESPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLISEADKRSRYGQLAQEYVR LSAEGRDVVAQVTGTREQQQLTEVIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT LEVSEGDRVRALGRELKGAIKAKEQFTLAGLENGAVRLRSGDRELRLPTERAVKLTHDYV EGTGAGTSASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPLAPEQAARKVESVSAVRL ASDQVRQSTGEANLDTAIQASRDRLMSDAEQAVSLAIPRAQQGQVHLSEITLLSEAVKSG QPLADVRTEIARQVNSGELIQLDSVSGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLM ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP QVIGLGPTHRAVHEMREAGVDARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR DMAELYQLVTAGGGRMVSSGDTAQLQAISTGQPFRLVQQRSAIDTVVMQEIVRQTPALRP AIESIIAGQVDTSLRLVDDVSPQQVPRQEGAWVPGNSVMEIRAPKKDQEQDTPAADEQTL TPEQLSLVRTDIIEAIRDDWMGRTPEAQLQTLVVAELNADRHAINDAIHAARHEKGDTGA EERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGES VLISPQQNTAQDISLFTPRDLTISHGDRVRFTRSDNDRGYVANSLWEVAGFTDDGAIRFR QGEQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVT LSRAKEHVQVYTDDLAGWSAGARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDKTA LGRRVLAENGLEGETMARFIAAGRKYPTPYVALPAWTRHGQEAGALLTEIRIEDDGMRVV LSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLLNA ARLTGGRITEPDEVARTVRSVAEVESAAKAEDPITLPPDEQQKLAEAQEKAARELAEQAR QELLPAVPGEESTRPEPLLSADEERRLRDGTARGERELDEAIQEAVAEGRGIRQQVREQM LRTEREWVVNVPEKDIELEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1323
Plasmid name	pKPC_UVA02
GenBank accession number	NZ_CP017929.1
Incompatibility group	IncFII(SARC14)
Genome size	113105 bp
Coordinate of oriT [Strand]	28729..29088 [+]
Drug resistance	_
Heavy-metal resistance	copper resistance; arsenical resistance; mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella oxytoca CAV1015 [571]

Reference

[1] Mathers AJ et al (2015) Klebsiella pneumoniae carbapenemase (KPC)-producing K pneumoniae at a single institution: insights into endemicity from whole-genome sequencing. Antimicrob Agents Chemother. 59(3):1656-63. [PMID:25561339]