 oriTDB accession number | 100861 |
| Name | oriT_pKPGJ-2c  |
Sequence Completeness  | intact |
| oriT length | 550 nt |
| oriT sequence | TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCG AAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGA GAGCGATATC |
| IR (inverted repeat) | [243-255] [257-269] nt (TATTTAATAATTC..GAATTATTAAATA) [311-318] [321-328] nt (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [337-338] nt |
| Conserved sequence flanking the nic site | GTAGTGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 933 |
| Name | Relaxase_pKPGJ-2c |
| GenBank accession number | WP_000130000 |
| Family | MOBC |
| Length | 101 aa |
| UniProt ID | A0A1Q1E5S3 |
| PDB ID | _ |
| Pfam | PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85 HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94 |
| Note | putative relaxase |
| Protein sequence [Download] | MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG |
Reference| ID | 1321 |
| Plasmid name | pKPGJ-2c |
| GenBank accession number | NZ_CP017852.1 |
| Incompatibility group | IncFII |
| Genome size | 66447 bp |
| Coordinate of oriT [Strand] | 45432..45981 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Klebsiella variicola GJ2 [244366] |
Reference