oriTDB

The information of the oriT region

oriTDB accession number	100860
Name	oriT_pEcoFMU073332a
Sequence Completeness	intact
oriT length	85 nt
oriT sequence	GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCT TAACCATGCGGCATCAGTGCAGATT
IR (inverted repeat)	_
Location of nic site	[58-59] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	D:58-59

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCT

61

TAACCATGCGGCATCAGTGCAGATT

Reference
N/A

I. Information of Relaxase

ID	932
Name	Nuclease_pEcoFMU073332a
GenBank accession number	WP_000635817
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.70E-18, Aligned region: 47..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKVLPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHNVDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1320
Plasmid name	pEcoFMU073332a
GenBank accession number	NZ_CP017845.1
Incompatibility group	ColRNAI
Genome size	5538 bp
Coordinate of oriT [Strand]	4938..5022 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli FMU073332 [562]

Reference
N/A