oriTDB
The information of the oriT region
oriTDB accession number 100860
Name oriT_pEcoFMU073332a  insolico
Sequence Completeness intact
oriT length 85 nt
oriT sequence GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCT TAACCATGCGGCATCAGTGCAGATT
IR (inverted repeat)_
Location of nic site [58-59] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
D:58-59
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 932
Name Nuclease_pEcoFMU073332a insolico
GenBank accession number WP_000635817
Family MOBP
Length 517 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.70E-18, Aligned region: 47..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKVLPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHNVDRGIAGKTPGRGE
RGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRNQDTGRNIAET
EREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ
EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1320
Plasmid name pEcoFMU073332a
GenBank accession number NZ_CP017845.1
Incompatibility group ColRNAI
Genome size 5538 bp
Coordinate of oriT  [Strand] 4938..5022 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli FMU073332 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU