oriTDB

The information of the oriT region

oriTDB accession number	100859
Name	oriT_pENH91
Sequence Completeness	intact
oriT length	591 nt
oriT sequence	TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)	[148-160] [166-178] nt (AGGCGCTGCCGGT..ACCGGAAGCGCCT) [250-255] [260-265] nt (GCCGGC..GCCGGC) [318-336] [339-357] nt (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site	[365-366] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	predicted by the oriTfinder
	L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG

61

TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC

121

GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG

181

AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT

241

CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA

301

GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA

361

TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC

421

TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA

481

AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC

541

GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA

Reference
N/A

I. Information of Relaxase

ID	931
Name	TraI_pENH91
GenBank accession number	WP_012478238
Family	MOB_P
Length	752 aa
UniProt ID	A0A1U9V3K4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNIEGQALALLKRGDEVMVMPID QATARRMSRIAVGDAVTITPKGSIKTSKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1319
Plasmid name	pENH91
GenBank accession number	NZ_CP017760.1
Incompatibility group	IncP(Beta)
Genome size	77172 bp
Coordinate of oriT [Strand]	30494..31084 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Cupriavidus necator NH9 [106590]

Reference
N/A