oriTDB
The information of the oriT region
oriTDB accession number 100859
Name oriT_pENH91  insolico
Sequence Completeness intact
oriT length 591 nt
oriT sequence TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)[148-160] [166-178] nt  (AGGCGCTGCCGGT..ACCGGAAGCGCCT)
[250-255] [260-265] nt  (GCCGGC..GCCGGC)
[318-336] [339-357] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [365-366] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 931
Name TraI_pENH91 insolico
GenBank accession number WP_012478238
Family MOBP
Length 752 aa
UniProt ID A0A1U9V3K4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ
DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF
QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP
GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ
HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA
AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNIEGQALALLKRGDEVMVMPID
QATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1319
Plasmid name pENH91
GenBank accession number NZ_CP017760.1
Incompatibility group IncP(Beta)
Genome size 77172 bp
Coordinate of oriT  [Strand] 30494..31084 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Cupriavidus necator NH9 [106590]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU