oriTDB

The information of the oriT region

oriTDB accession number	100852
Name	oriT_pKPGJ-3d
Sequence Completeness	core
oriT length	106 nt
oriT sequence	AGTATGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCATCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)	[18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCATCCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:18-35;R:41-58

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTATGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCATCCAAAAACGT

61

CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Reference
N/A

I. Information of Relaxase

ID	924
Name	Putative relaxase of pKPGJ-3d
GenBank accession number	WP_077138896
Family	MOB_P
Length	659 aa
UniProt ID	A0A1Q1E692
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.50E-31, Aligned region: 13..334
Note	putative relaxase
Protein sequence [Download]	MVIPKIIEGRRDKKSSFAQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIR KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSW PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS FSKDTLHQACRLLELKNGWSHSNGAYVVNERQQIVRNPHSKKERGNWRSLDRINKMENKE GVETLYRYIVGDEQVGGSRQNFIHVSAGLREAKSWDDVHKTFADIGLRFEKAQGKKGYVI THEHQNLKTAVKASLVFNKAQYTLKSMEERFGEYQPSRIEPAKVSVFKTAYTPGAYRRDA NKRLQRKIERAEERMLLKGRYRAYRNNLPTYSPDKDRIADEYRKIAQHTRLVKNNVRHSV SDPYTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSLRVDGDGEFKELCCN RIVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSENDDKFKPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1312
Plasmid name	pKPGJ-3d
GenBank accession number	NZ_CP017288.1
Incompatibility group	IncL/M(pOXA-48)
Genome size	58665 bp
Coordinate of oriT [Strand]	14402..14507 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella variicola GJ3 [244366]

Reference

[1] Di DY et al (2017) Emergence of Klebsiella variicola positive for NDM-9, a variant of New Delhi metallo-β-lactamase, in an urban river in South Korea. J Antimicrob Chemother. 72(4):1063-1067. [PMID:28087584]