oriTDB
The information of the oriT region
oriTDB accession number 100851
Name oriT_pKPGJ-3c  insolico
Sequence Completeness intact
oriT length 550 nt
oriT sequence TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCG AAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGA GAGCGATATC
IR (inverted repeat)[243-255] [257-269] nt  (TATTTAATAATTC..GAATTATTAAATA)
[311-318] [321-328] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [337-338] nt
Conserved sequence flanking the
  nic site
GTAGTGTGT|GG
Note predicted by the oriTfinder
L:243-255;R:257-269;L:311-318;R:321-328;D:337-338
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 923
Name Relaxase_pKPGJ-3c insolico
GenBank accession number WP_000130000
Family MOBC
Length 101 aa
UniProt ID A0A1Q1E5S3
PDB ID _
Pfam PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85
HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94
Note putative relaxase
Protein sequence [Download] MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS
RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1311
Plasmid name pKPGJ-3c
GenBank accession number NZ_CP017287.1
Incompatibility group IncFII
Genome size 73385 bp
Coordinate of oriT  [Strand] 55248..55797 [-]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella variicola GJ3 [244366]
Reference
[1] Di DY et al (2017) Emergence of Klebsiella variicola positive for NDM-9, a variant of New Delhi metallo-β-lactamase, in an urban river in South Korea. J Antimicrob Chemother. 72(4):1063-1067. [PMID:28087584]